Fig 1: Knockdown of XIST alleviates iron overload-induced pancreatic islets/beta cells injury in T2D rats. ((a) and (b)) The gene expression was detected by RT-qPCR. (c) The expression of ALK2 protein was detected by Western blot. (d) The body weight of each group rats. (e) Comparison of postprandial and fasting blood glucose concentration of differently treated rats. ((f), (g), (h)) Comparison of iron, TIBC, and ferritin constituent in serum from each group. (i) Comparison of iron metabolic-related proteins of differently treated rats. (j) The H&E and insulin staining in pancreatic tissues. Scale bar = 100 µm. *P < 0.05, **P < 0.01.
Fig 2: miR-130a-3p directly binds to and regulates the expression of ALK2. (a) The predicted binding sites of miR-130a-3p to ALK2 sequence using StarBase. ((b) and (c)) The expression of ALK2 protein was detected by Western blot. (d) The relative luciferase of HEK 923 cells was transfected with miR-130a-3p mimics or inhibitors and luciferase constructs of WT- or MUT-ALK2-UTR. **P < 0.01.
Fig 3: XIST promotes iron overload-induced INS-1 cells injury by regulating miR-130a-3p/ALK2 axis. (a) The expression of ALK2 protein was detected by Western blot. (b) Cell proliferation was measured by MTT assay. (c) Representative results of TUNEL staining for every group. Scale bar = 100 µm. (d) Knockdown of XIST downregulated LIP. (e) GSIS assay was used to measure the secretion of insulin. (f) The protein expression was measured by Western blot. *P < 0.05, **P < 0.01.
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